Chick Embryo Fibroblasts and Dorsal Root Ganglion

I have been dissecting chick embryos this whole week for the primary culture of their fibroblasts, dorsal root / spinal ganglion (cluster of nerve cell bodies), and cardiac cells. In these fluorescence microscopy images, the fibroblasts appear to have an elongated, triangular, heterogenous morphology while the ganglion have a shiny ring around their cell body with thin long protrusions which are the neurites (axons or dendrites).

In Dr. Mason’s words, “DRG cells are special. Process that goes to skin is dendritic and the one to CNS is an axon. But both look like axons.” That is, DRGs are pseudounipolar cells – they have two axons instead of having an axon and dendrite. One axon extends centrally toward the spinal cord; the other axon extends toward the skin or muscle.

CEF and DRG 3CEF and DRG 2

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